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Dr. Om Prakash Sharma, Ph.D.
Designation: Scientist 'D'
Email: [email protected], [email protected]
Tel : +91-020-25329036
EDUCATIONAL QUALIFICATION:
- Ph. D. (2006) : Microbiology (University of Delhi, India)
- M. Sc. (2000) : Microbiology (Dr. R.M.L. University, Faizabad,India)
- B. Sc. (1995) : Botany, Zoology,Chemistry (Dr. R.M.L. University, Faizabad, India).
PROFESSIONAL EXPERIENCE :
- Nov.-2011 to present: Scientist-“C”, National Centre for Cell Sciences, Pune 411007, Maharashtra,India.
- Sep.-2011 to Nov.-2011: Research Associate, Schools of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332-0230, USA.
- Oct.-2007 to Aug.-2011: Postdoctoral Research Fellow, Department of Earth Ocean and Atmospheric Science Florida State University, Tallahassee, FL, USA.
- Jan.-2007 to Oct.-2007: Research Associate, Molecular biology laboratory Department of Zoology,University of Delhi.
- May-2006 to Jul.-2006: Visiting Fellow, Environmental Biotechnology Department, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland.
- Jan.-2001 to June-2001: Project Assistant, Agricultural Microbiology Laboratory National Botanical Research Institute (NBRI) Lucknow.
- Jul.-2000 to Jan.-2000: Project Assistant, Plant Pathology Laboratory National Botanical Research Institute (NBRI) Lucknow.
FELLOWSHIP/AWARDS:
- Selected as Fellow of Indian National Science Academy (INSA) in Environmental Microbiology to develop the Indo-Israel bilateral program in wastewater treatment for the year 2016.
- Received “Young Achiever Award” for significant contribution in agricultural microbiology in 2014 from Institute of Agricultural Science, Banaras Hindu University, Varanasi
- July-2003 to July-2006: Senior Research Fellow, Council of Scientific and Industrial Research (CSIR,Govt. of India) Department of Zoology,University of Delhi.
- July-2001 to July-2003: Junior Research Fellow, Council of Scientific and Industrial Research (CSIR,Govt. of India), Department of Zoology, University of Delhi.
- **Qualified national level “Graduate Aptitude Test in Engineering (GATE)” with 90 percentile.
- **Qualified for visiting fellow positions in Canadian Government’s Laboratories under NSERC Scheme.
RESEARCH INTERESTS:
- Bacterial Systematics; Environmental Microbiology; Microbial Ecology; Anaerobic Microbiology; Bacterial Physiology and Metabolism.
PUBLICATIONS:
- Hemme CL, Green SJ, Rishishwar L, Prakash O, Pettenato A , Chakraborty R, Deutschbauer A, Nostrand JD Van, Wu L, He Z, Jordan KI, Hazen TC , Arkin AP, Kostka JE.(2016) Lateral Gene Transfer in a Heavy Metal-Contaminated Groundwater Microbial Community. mBio02234-15 (In Press IF 6.7)
- Prakash, O., Sharma, A., Nimonkar, Y., Shouche Y. S. (2015): Proposal for creation of a new genus Neomicrococcus gen. nov. for Zhihengliuella aestuarii and Micrococcus lactis (Chittpurna et al., 2011). Int J Syst Evol Microbiol doi: 10.1099/ijsem.0.000490. ISSN 1466-5026 (IF 2.8).
- Prakash, O., Nimonkar, Y., Vaishampayan, A., Mishra, M., Kumbhare, S., Josef, N., Yogesh Shouche, YS, (2015). Description of Pantoea intestinalis sp. nov. : isolated from Human gut.' Int. J. Syst. Evol Microbiol doi: 10.1099/ijsem.0.000419. ISSN 1466-5026(IF 2.8).
- Prakash O., Pansare A & Dhar SK (2015). Bioprospecting Microbial Diversity: IPR Issues. In Intellectual Property Issues in Biotechnology. CABI, UK (In Press).
- Prakash, O., Sharma, R, Singh, P., Yadav, A. (2015). Strategies for taxonomical characterization of agriculturally important microorganisms. In. Microbial Inoculants in Sustainable Agricultural Productivity. Springer India (In Press).
- Prakash, O., and Jangid K. (2015). Preservation of uncultivated microbial cells for single cell genomics and cultivation in future. Single Cell Biology S1:003. doi:10.4172/2168-9431.S1-003.
- Prakash, O., Nimonkar, Y., Shaligram,S., Joseph, N., Shouche, YS (2015). Cellular fatty acids response to environmental stresses in endophytic Micrococcus spp. Ann. Microbiol (in press)
- Prakash, O., Sharma, R., Rahi, P., & Karthikeyan, N. (2015). Role of Microorganisms in Plant Nutrition and Health. In Nutrient Use Efficiency: from Basics to Advances (pp. 125-161). Springer India.
- Prakash, O., Nimonkar, Y., Munot, H., Sharma, A., Vemuluri, V. R., Chavadar, M. S., Shouche Y. S. (2014): Description of Micrococcus aloeverae sp. nov. : an endophytic actinobacterium isolated from leaf of Aloe barbadensis. Int J Syst Evol Microbiol, doi:10.1099/ijs.0.063339-0 (Corresponding author, IF 2.8).
- Sharma, A., Dhar, S. Prakash, O., Vemuluri, V. R., Thite, V., Shouche, Y. S. (2014): Description of Domibacillus indicus sp. nov., isolated from ocean sediments of Lakshadweep and emended description of the genus Domibacillus. Int J Syst Evol Microbiol, doi:10.1099/ijs.0.064295-0 (IF 2.8).
- Prakash,O., Munot, H, Nimonkar,Y, Sharma,M, Kumbhare,S, Shouche,YS (2014) Description of Pelistega indica isolated from Human gut. Int J Syst Evol Microbiol(doi: 10.1099/ijs.0.059782-0. IF 2.3).
- Jasrotia,P., Green, SJ, Canion, A., Overholt, W., Prakash, O, Wafula,D, Hubbard, D., Watson,D, Schadt, C, Brooks,S and Kostka JE(2014). Watershed scale fungal community characterization along a pH gradient in a subsurface environment co-contaminated with uranium and nitrate. Appl. Environ. Microbiol. (doi: 10.1128/AEM.03423-1 IF 3.8).
- Overholt, WA, Green, SJ, Marks,KP, Venkatraman, R. , Prakash, O, and Kostka, JE(2013). Draft Genome Sequences for Oil-Degrading Bacterial Strains from Beach Sands Impacted by the Deepwater Horizon Oil Spill. genomA (Vol 1 Issue 6 e01015-13).
- Venkatramanan R., Prakash O., Woyke T., Chain P. S. G., Goodwin L. A , Watson D. B., Brooks S. C. , Kostka J. E. and Green S. J. (2013) Genome sequences for three denitrifying bacterial strains isolated from a uranium- and nitrate-contaminated subsurface environment. genomeA. e00449-13. doi: 10.1128/genomeA.00449-13.
- Green S., Rishishwar L., Prakash O., Jordan I., Kostka J. (2013) Insights into environmental microbial denitrification from integrated metagenomic, cultivation and genomic analyses. In: Nelson K. (Ed.) Encyclopedia of Metagenomics: SpringerReference (www.springerreference.com). Springer-Verlag Berlin Heidelberg, 2013. DOI: 10.1007/Springer Reference_359242 2013-03-15 15:03:44 UTC (Book chapter)
- Prakash O., Nimonkar, Y., Shouche, Y.S. (2013) Practice and prospects of microbial preservation. FEMS Microbiol Lett. 339:1-9.
- Prakash O., Shouche, Y., Jangid, K., Kostka, J.E. (2013) Microbial cultivation and the role of microbial resource centers in the omics era. Appl Microbiol Biotechnol. 97:51-62.
- Canion, A., Prakash O., Green, S.J., Jahnke, L., Kuypers, M. M., Kostka, J.E. (2013). Isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold Arctic fjord sediments (Svalbard, Norway). Environ Microbiol. DOI: 10.1111/1462-2920.12110 (equal authorship).
- Prakash O. and Lal, R. (2013). Role of Unstable Phenanthrene Degrading Pseudomonas species in the Natural Attenuation of Phenanthrene Contaminated Site. Korean J. Microbiol Biotechnol. 41: 1-9. Korean
- Prakash O., Jangid, K. Shouche, YS(2013). Carl Woese: From Biophysics to Evolutionary Microbiology. Ind. J. Microbiol. (accepted).
- Kostka, J., Green, S., Rishishwar, L., Prakash O., Katz, L., Marino-Ramirez, L., Jordan, K., Munk, C., Ivanova, N., Mikhailova, N., Watson, D., Brown, S., Palumbo, A. and Brooks, S. (2012). Genome sequences for six Rhodanobacter strains isolated from soils and the terrestrial subsurface with variable denitrification capabilities. J. Bact. 194: 4461
- Lin, X., Green, S., Tfaily, M., Prakash O., Konstantinidis, K., Corbett, J., Chanton, J. and Cooper W. and Kostka, J. (2012). Microbial community structure and activity linked to contrasting biogeochemical gradients in bog and fen environments of the Glacial Lake Agassiz Peatland. Appl. Environ. Microbiol. 78: 7023-7031.
- Prakash O., Green, S. J., Jasrotia, P., Overholt, W. A., Canion, A., Watson, D. B., Jardine, P. M., Brooks, S. C. and Kostka, J. E. (2012). Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer. Int J Syst Evol Microbiol 62: 2457–2462.
- Green, S. J., Prakash O., Jasrotia, Overholt, W. A., Cardenas, E., Hubbard, D., Tiedje, J. M., Watson, D. B., Jardine, P. M., Brooks, S. C. and Kostka, J. E. (2012). Denitrifying Bacteria from the Genus Rhodanobacter Dominate Bacterial Communities in the Highly Contaminated Subsurface of a Nuclear Legacy Waste Site Appl. Environ. Microbiol.78: 1039-1047.
- Kostka, J. E., Prakash O., Overholt, W., Green, S., Freyer, G., Canion, A., Delgardio, J., Norton, N. and Huettel, M. (2011). Hydrocarbon-degrading bacteria and the bacterial community response in Gulf of Mexico beach sands impacted by the Deepwater Horizon oil spill. Appl. Environ. Microbiol. 77, 7962-7974.
- Prakash O., Gihring, T. M, Dalton, D. D., Chin, K. J., Green, S. J., Akob, D. M.,Wanger, G. and Kostka, J. E. (2010). Geobacter daltonii sp. nov., an Fe(III)- and uranium(VI)-reducing bacterium isolated from the shallow subsurface exposed to mixed heavy metal and hydrocarbon contamination. Int J Syst Evol Microbiol 60, 546-553.
- Green, S. J., Prakash O., Akob, D. M., Gihring, T. M., Jardin, P., Watson, D. B., and Kostka, J. E. (2010). Denitrifying bacteria isolated from terrestrial subsurface sediment exposed to mixed contamination. Appl Environ Microbiol 76, 3244-3254. (Equal authorship).
- Dadhwal, M., Singh, A., Prakash O., Gupta, S. K., Kumari, K., Sharma, P., Jit, S., Verma, M., Holliger, C. and Lal, R. (2009). Proposal of biostimulation for hexachlorocyclohexane (HCH)-decontamination and characterization of culturable bacterial community from high-dose point HCH-contaminated soils. J Appl Microbiol 106, 381-92.
- Gupta, S. K., Kumari, R., Prakash O. and Lal, R. (2008). Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site. Int J Syst Evol Microbiol 58, 1339-1345.
- Jit S., Prakash O. and Lal R. (2008). Flavobacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil, Int J Syst Evol Microbiol 58, 1665-1669.
- Raina, V., Suar, M., Singh, A., Prakash O., Dadhwal, M., Gupta, S. K. and Lal, R. (2008). Enhanced biodegradation of hexachlorocyclohexane (HCH) in contaminated soil via inoculation of Sphingobium indicum B90A. Biodegradation 19, 27-40.
- Prakash O., Kumari, K. and Lal, R. (2007). Pseudomonas delhiensis sp. nov, from a fly ash dumping site of a thermal power plant. Int J Syst Evol Microbiol 57, 527-531.
- Prakash O. and Lal, R. (2006). Description of Sphingobium fuliginis sp. nov., a phenanthrene degrading bacterium from fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov. Int J Syst Evol Microbiol 56, 2147-2152.
- Pal, R., Bala, S., Dadhwal, M., Kumar, M., Dhingra, G., Prakash O., Prabagaran, S. R., Shivaji, S., Cullum, J., Holliger, C. and Lal, R. (2005). Hexachlorocyclohexane degrading bacterial strains Sphingomonas paucimobilis B90A, UT26 & Sp+, having similar lin genes represent three distinct species, Sphingobium indicum sp. nov., Sphingobium japonicum sp. nov. and Sphingobium francense sp. nov. and reclassification of [Sphingomonas] chungbukensis as Sphingobium chungbukense comb. nov. Int J Syst Evol Microbiol 55, 1965-1972.
- Prakash O., Suar, M., Raina, V., Dogra C., Pal, R. and Lal, R. (2004). Residues of hexachlorocyclohexane isomers from soil and water samples of Delhi and adjoining areas. Curr. Sci. 87: 73-77.
- Prakash O., Verma, M., Kumar, M., Singh, A., Gupta S. K. and Lal R. (2007). Polyphasic Approach: A More Realistic Way of Microbial Classification. Ind. J. Microbiol. 47: 98-108.
- Lal R., Jit S., Verma M., Dadhwal M, Singh A., Prakash O., Sharma P. and Khanna M. (2006). Nanoorganisms: Smallest form of life got smaller:! Ind J Microbiol 46, 413.
- Lal, R., Dadhwal, M., Singh, A., Prakash O. Malhotra, S. and Sharma, P. (2005). Hexachlorocyclohexane (HCH) residues in Indian environment: Problems and solution. Published in Proceedings of 8th International HCH and pesticide forum, 26-28 May 2005, Sofia, Bulgaria.
- Lal, R., Pal, R., Dogra, C., Prakash O., Malhotra, S., Sharma, P., Suar, M. and Raina V. 2003. Problem Posed by Hexachlorocyclohexane (HCH) Residues in India and Need for Developing Bioremediation Technologies. Published in Proceedings of International Symposium organized in Pune India.
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PAPERS PRESENTED IN NATIONAL AND INTERNATIONAL CONFERENCES:
- Kostka, J. E., Green, S. J., Prakash, O., Jasrotia, P., Rishishwar, L., Schadt, C., Watson, D. Brooks, S. (2012). Nitrate Attenuation and the Impact of pH on the Predominant Denitrifying Microbial Groups in the OR-IFRC Site Subsurface (OR-IFRC, PI-Meeting).
- Lin, X., Green, S., Tfaily, M. M., Prakash, O., Konstantinidis, K, Corbett, J. E., Chanton, J. P., Cooper, W. and Kostka, J. E. (2012). Microbial community structure and activity linked to contrasting biogeochemical gradients in bog and fen environments of the Glacial Lake Agassiz Peatland. (Jun16-19, ASM2012, San Francisco.USA).
- Green, S. J., Prakash, O., Jasrotia, P., Jordan, I. K., Katz, L.S., Rishishwar, L., Schadt, C. W., Brooks, S. C. and Kostka, J. E. (2012). Genomic and physiological characterization of bacteria from the genus Rhodanobacter. (Jun16-19, ASM2012, San Francisco, USA).
- Kostka, J. E., Lin, X., Chanton, P. R., Green, S. J., Tfaily, M. M., Prakash, O., Chanton, J. P., Cooper, W., Steinweg, J. M. and Schadt C. W. (2012). The response of soil carbon storage and microbially mediated carbon turnover to simulated climatic disturbance in a northern peatland forest: revisiting the concept of soil organic matter recalcitrance. (TES- Meeting)
- Overholt, W.A., Green, S.J., Prakash, O., Gihring, T. M., Akob, D. M., Jasrotia, P., Jardine, P. M., Watson, D. B., Brown, S. D., Palumbo, A. V., Sul, W. J., Tiedje, J., Brooks, S., Kostka, J. E. (2010). Denitrifying bacteria from the Genus Rhodanobacter are key members of microbial communities in the acidic, uranium-contaminated subsurface at Oak Ridge, TN. 109th General Meeting of the American Society for Microbiology, Philadelphia, PA.
- Green, S. J., P. Jasrotia, D. Hubbard, O. Prakash, T. M. Gihring, D. M. Akob, P. M. Jardine, D. B. Watson, S. D. Brown, A. V. Palumbo, and W. J. Sul, J. Tiedje, and J. E. Kostka. 2010. Current Molecular Methods that Target Denitrification Genes Do Not Detect Key Denitrifying Taxa. International Society for Microbial Ecology Symposium, Seattle, WA USA.
- Prakash, O., Overholt, W., Green, S., Freyer, G., Canion, A., Delgardio, G., Norton, N., Huettel, M., and Kostaka, J. E. (2011). Isolation, Enumeration, and Characterization of Oil-Degrading Bacteria and Fungi from Oiled Gulf Sediments Impacted by the Deepwater Horizon Spill. 111th General Meeting of the American Society for Microbiology, New Orleans, Louisiana.
- Green, S. J., Jasrotia, P., Overholt, W. A., Prakash, O., Gihring, T. M., Jardine, P. M., Watson, D. B., Brown, S. D., Palumbo, A. V., Scheldt, C., Brooks, S., Sul, W., J., Tiedje, J., Reed, J. and Kostka, J. E. (2011). Why are Rhodanobacter spp. so dominant in a uranium-contaminated subsurface environment? 111th General Meeting of the American Society for Microbiology, New Orleans, Louisiana.
- Canion, A. K., Prakash, O., Green, S. J., & Kostka, J. E. (2010). Characterization of N2-Producing Bacterial Communities from Arctic Sediments. 2010 State of the Arctic Conference: Miami, Florida.
- Jasrotia, P., Prakash, O., Canion, A. K., Green, S. J. and Kostka J. E. (2010) Isolation and Characterization of acid tolerant denitrifying bacteria and fungi from the terrestrial subsurface DOE-SBR 5th Annual PI Meeting 2010 March 29-31, 2010 JW Marriott, Washington, DC
- Jasrotia, P., Canion, A. K., Prakash, O., Green, S. J., and Kostka, J. E. (2010). Isolation and characterization of denitrifying fungi and bacteria from low pH, nitrate- and uranium- contaminated groundwater. Presented in Dept. of Oceanography, Graduate symposium.
- Kostka, J.E., Prakash, O., Green, S.J., Jasrotia, P., Kerkhof, L., Chin, K.-J., Keller, M., Venkateswaran, A., Elkins, J.G., Stucki, J.W. [2009]. Structure and Function of Subsurface Microbial Communities Affecting Radionuclide Transport and Bioimmobilization. Department of Energy Environmental Remediation Sciences Program 4th Annual PI Meeting. Landsdowne, VA.
- Spalding, B., Watson, D., Jardine, P., Zhang, F., Tang, G., Parker, J., Kostka, J.E., Green, S.J., Prakash, O., Wu, W.-M., Zhang, G., Kelly, S., Mehlhorn, T., Carley, J., Kemner, K., Criddle, C., Schadt, C., Luo, W., Gu, B., Hortia, J. and Brooks, S. [2009]. Site-Wide Oak Ridge FRC Watershed Monitoring of Contaminant Distribution and Attenuation Processes. Department of Energy Environmental Remediation Sciences Program 4th Annual PI Meeting. Landsdowne, VA.
- Prakash, O., Green, S.J., Gihring, T., Jardine, P., Watson, D. and Kostka, J.E. [2009] Denitrification activity and novel denitrifying bacteria isolated from a terrestrial subsurface aquifer exposed to mixed contamination. 109th General Meeting of the American Society for Microbiology, Philadelphia, PA.
- Kostka, J. E., H. J. Mills, D. M. Akob, O. Prakash, D. Swofford, L. Kerkhof, K.-J. Chin, M. Keller, J. Elkins, and J. W. Stucki. 2008. Structures and Function of Subsurface Microbial Communities Affecting Radionuclide Transport and Bioimmobilization. DOE 3rd Annual ERSP Spring PI Meeting, Lansdowne, VA.
- Poster presented in International Symposium on 'Utilization of Microbes for the Development of Bioremediation Technologies' organized at the Department of Zoology, University of Delhi, Delhi. Sponsored by SDC, Switzerland and DBT, India (October 7, 2002 to October 8, 2002).
- Poster presented in International Symposium on 'Molecular Toxicology and Environmental Health' organized by ITRC Lucknow (November 5-8/ 2003).
- Poster presented. in 46th AMI (Association of Microbiologist of India) Annual Conference 'Microbiotech 2005', organized by Microbiology Department of Osmania University Hyderabad (A.P.).
- Poster presented in 47th AMI (Association of Microbiologist of India) Annual Conference 2006, organized by Department of Biotechnology Barkatullah University, Bhopal (M.P.) India.
TRAINING COURSES/WORKSHOPS
- Attended the “U. S. EPA/ORD grant process workshop- how it works” jointly organized by United State Environmental Protection Agency (USEPA) and British-Petroleum (BP) at Florida Agricultural and Mechanical University (FAMU), Tallahassee, FL, USA December-09-2010.
- Attended professional development workshop on “Contracts and Grants, from Cradle to Grave” organized by “The Graduate School” Florida State University,Tallahassee, FL, USA (Oct.-28, 2010).
- Attended professional development workshop on “Writing Scientific Papers” organized by “The Graduate School” Florida State University,Tallahassee, FL, USA on November 9 & 10, 2010.
- Received training of “Radiation Safety Short Course” organized by Department of Environmental Health and Safety, Florida State University” Tallahassee FL, USA on June, 4,11 &18, 2008.
- Received a three months summer training on “Isolation and Identification of Plant Pathogenic Bacteria” from plant pathology division of “National Botanical Research Institute (NBRI), Lucknow, India (May-1999 to July-1999).
- Participated in CSIR sponsored winter workshop on “Tool and Techniques of Plant Diversity Assessment, Conservation and Inventorization” organized by CIMAP Lucknow (16th Jan.2000 to 4th Feb 2000).
- Completed an eleven months project work on “Biological Control of Plant Diseases” from “National Botanical Research Institute (NBRI), Lucknow, India (July-2000 to June-2001).
- Completed a nine months project work on “Bioremediation of Nitroaromatic Compounds” from Microbial Type Culture Collection (MTCC) division of Institute of Microbial Technology, Chandigarh, India. (July-2001to April-2002).
STUDENTS MENTORED:
- Nicole Roberts: Biology undergraduate,Department of Oceanography Florida State University
- Zoe Bider-Canfield: Department of Oceanography Florida State University,Tallahassee, FL
- Patrick Chanton: Biology graduate, Georgia Institute of Technology, Atlanta,GA.
AFFILIATIONS/MEMBERSHIP :
- American Society for Microbiology(Annual)
TEACHING EXPERIENCE
- Taught microbiology classes of graduate and under-graduate students at Florida State University,Tallahassee, FL, USA.
POSTDOCTORAL ADVISOR:
- Prof. Joel E. Kostka (Editor, Applied and Environmental Microbiology)
- Department of Biology, Georgia Institute of Technology, Atlanta, GA.
DOCTORAL ADVISOR:
- Prof. Rup Lal (Editor in chief, Indian Journal of Microbiology)
- Department of Zoology, University of Delhi, Delhi,India.